کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
4365080 1616341 2012 7 صفحه PDF سفارش دهید دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification and characterisation of a Bacillus licheniformis strain with profound keratinase activity for degradation of melanised feather
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موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست علوم زیست محیطی (عمومی)
پیش نمایش صفحه اول مقاله
Identification and characterisation of a Bacillus licheniformis strain with profound keratinase activity for degradation of melanised feather
چکیده انگلیسی

Significant amount of keratins in the form of feather, hair, hoof and horn are generated annually by the livestock industry. Keratinases are increasingly important in the reprocessing and environmental pollution control of keratin wastes. The aim of this study is to isolate a microbial strain of high keratinase activity and to evaluate its feather degrading potential. Thirty-two keratin degrading microbial strains from farmyard wastes and primary effluent were isolated using a selective medium containing feather meal at 30, 37 and 50 °C. One of the isolates, which demonstrated the highest keratinolytic activity (11.00 ± 0.71 U ml−1) was identified as a species of Bacillus licheniformis based on the 16S rDNA analysis, designated as strain N22 and deposited in a culture collection. Optimum keratinase production by this bacterium was achieved in 32 h using a minimum growth medium containing 1.1% (w/v) feather meal at 50 °C and pH 8.5. The molecular weight of the keratinase was ≈28 kDa as determined using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis and confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The keratinase reported here significantly degraded melanised feather in 48 h in the absence of reducing agents. There are few reports on the evaluation of feather degrading ability of keratinases using highly resistant melanised feather. The efficient degradation of melanised feathers by this keratinase may offer an environmentally friendly solution to the degradation of feather waste and other organic matter of similar molecular composition.


► We report the isolation of a new strain of Bacillus licheniformis from farmyard waste.
► Its keratinase degraded melanised feather in the absence of reducing agents.
► Degradation conditions (50 °C, pH 8.5) suitable for biotechnological applications.
► Offers environmentally friendly solution to the degradation of feather waste.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Biodeterioration & Biodegradation - Volume 74, October 2012, Pages 54–60
نویسندگان
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