کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4365137 | 1616345 | 2012 | 6 صفحه PDF | دانلود رایگان |
Five wool-degrading bacterial strains were selected from a set of 115 isolates from a less conventional source, i.e., the wool of Portuguese Merino sheep, based on their promising keratinolytic abilities. All selected strains belong to the genus Bacillus, probably Bacillus subtilis/licheniformis, according to 16S rRNA sequencing. They were able to grow on and hydrolyse feathers and wool (in both native and milled forms), and human hair and nails to a lesser extent. The maximum keratinase activity was recorded on milled chicken feathers. Biodegradation of such keratin-rich matrices increased the amount of soluble proteins in the fermentation broth and implied the action of extracellular enzymes from those adventitious microorganisms. Therefore, such novel strains have a potential for effective use in solid waste management strategies encompassing keratin-rich materials based on submerged fermentation. Concomitantly, the nutritional value of the broth may be improved for eventual formulation of animal feed.
► Five wool-degrading bacterial strains selected with notable keratinolytic activity.
► All strains able to grow and hydrolyse feathers and wool, both native and milled.
► Proteolytic stability observed over wide range of pH and temperature.
► Strains with potential for upgrading keratin-rich solid wastes.
Journal: International Biodeterioration & Biodegradation - Volume 70, May 2012, Pages 60–65