کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4365303 1301751 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A new Bauhinia monandra galactose-specific lectin purified in milligram quantities from secondary roots with antifungal and termiticidal activities
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست علوم زیست محیطی (عمومی)
پیش نمایش صفحه اول مقاله
A new Bauhinia monandra galactose-specific lectin purified in milligram quantities from secondary roots with antifungal and termiticidal activities
چکیده انگلیسی

This work describes the purification in milligram quantities of a lectin from Bauhinia monandra secondary roots (BmoRoL) and its antifungal and termiticidal activities. The BmoRoL (6.2 mg) was isolated through ammonium sulfate fractionation and affinity chromatography on guar gel. Native lectin was resolved as a single band on polyacrylamide gel electrophoresis for basic proteins. Under denaturing and reducing conditions it appeared as a unique glycosylated polypeptide of 26 kDa. The highest agglutination activity of BmoRoL was found with glutaraldehyde-treated rabbit erythrocytes. BmoRoL showed antifungal activity against phytopathogenic species of Fusarium and was more active on Fusarium solani. The lectin also showed termiticidal activity on Nasutitermes corniger workers and soldiers with LC50 of 0.09 and 0.395 mg ml−1 for 12 days. In conclusion, BmoRoL is a new antifungal and termiticidal lectin that can be purified in milligram quantities and has potential biotechnological application for control of agricultural pests.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Biodeterioration & Biodegradation - Volume 65, Issue 5, August 2011, Pages 696–702
نویسندگان
, , , , , , , , ,