کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4365333 1301754 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Bioremediation of volatile oil hydrocarbons by epiphytic bacteria associated with American grass (Cynodon sp.) and broad bean (Vicia faba) leaves
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست علوم زیست محیطی (عمومی)
پیش نمایش صفحه اول مقاله
Bioremediation of volatile oil hydrocarbons by epiphytic bacteria associated with American grass (Cynodon sp.) and broad bean (Vicia faba) leaves
چکیده انگلیسی

Fresh leaves of American grass and broad beans grown in pristine soil were naturally colonized with cultivable volatile oil hydrocarbon-utilizing bacteria, whose numbers increased significantly in plants grown in oily soil. According to their 16S rRNA gene sequences those bacteria were affiliated to various species of the genera Rhodococcus and Pseudomonas. Qualitative growth studies revealed that pure cultures of these phyllospheric bacteria could grow successfully on a solid mineral medium containing individual alkanes with chain lengths of C9 through C40 and the aromatics phenanthrene, naphthalene, and biphenyl as sole sources of carbon and energy. Quantitative measurements showed that the individual pure bacterial isolates degraded between about 20 and 30% of crude oil, n-hexadecane, or phenanthrene in batch culture after a one-week incubation. These results reflect the high hydrocarbon degradation potential of those bacteria. The isolates were diazotrophic (nitrogen fixers), meaning that they were self-dependent in covering their nitrogen requirements. Incubating fresh leaves in closed microcosms containing volatile oil hydrocarbons resulted in up to more than 80% attenuation of these compounds after two weeks. Experimental evidence was provided that the leaf tissues did not contribute to this attenuation, which was exclusively due to the bacterial activity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Biodeterioration & Biodegradation - Volume 65, Issue 6, September 2011, Pages 797–802
نویسندگان
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