کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4365980 1301797 2008 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Bacterial diversity associated with archaeological waterlogged wood: Ribosomal RNA clone libraries and denaturing gradient gel electrophoresis (DGGE)
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست علوم زیست محیطی (عمومی)
پیش نمایش صفحه اول مقاله
Bacterial diversity associated with archaeological waterlogged wood: Ribosomal RNA clone libraries and denaturing gradient gel electrophoresis (DGGE)
چکیده انگلیسی

The identity of bacteria in waterlogged archaeological wood responsible for degradation was investigated using novel isolation techniques and molecular DNA technology. Wood in cultural heritage and archaeological sites is vulnerable to bacterial attack, with bacterially driven wood-decay predominating when wood is buried in sediments. In the research presented, DNA was extracted directly from wood samples and the diversity of bacterial species was determined. Using cultures isolated by project partners from archaeological wood of the same origin at 19 different European sites, a comparison of the DNA of nine culturable isolates and DNA from species commonly appearing in 56 independent molecular fingerprints was made. Results show that sequences representing bacteria from the Cytophaga-Flavobacterium-Bacteroides (CFB) complex and the Pseudomonas group were commonly recovered, with relatives of the Cellvibrio and Brevundimonas groups also present. These observations are the first to provide a molecular link between environmental samples where degradation had occurred and cultured organisms, although the precise role and capability of the bacterial strains remains obscure.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Biodeterioration & Biodegradation - Volume 61, Issue 1, January 2008, Pages 106–116
نویسندگان
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