کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4366063 | 1301813 | 2006 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Construction of a genetically engineered microorganism for degrading organophosphate and carbamate pesticides
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
علوم زیست محیطی (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Construction of a genetically engineered microorganism for degrading organophosphate and carbamate pesticides Construction of a genetically engineered microorganism for degrading organophosphate and carbamate pesticides](/preview/png/4366063.png)
چکیده انگلیسی
Genetically engineered microorganisms (GEMs) have shown potential for enhanced degradation of a range of substrates. In this study, a DNA fragment including the open reading frame of mpd (methyl parathion hydrolase encoding gene) and cognate regulator of a methyl parathion (MP)-degrading strain Pseudomonas putida DLL-1 was cloned by the shotgun method. The fragment was cloned into a broad-host vector pBBR1MCS-2 to produce a recombinant plasmid pBBR-mpd. CDS-pBBR-mpd, a GEM, was successfully constructed by transforming it into a carbofuran-degrading strain Sphingomonas sp. CDS-1, and it degraded both carbofuran and MP. CDS-pBBR-mpd maintained stable and high MP hydrolase activity (50.72 nmol minâ1 μgâ1 protein, which was 6.57 times higher than that of P. putida strain DLL-1). and appears to be a promising GEM for environmental bioremediation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Biodeterioration & Biodegradation - Volume 58, Issue 2, September 2006, Pages 65-69
Journal: International Biodeterioration & Biodegradation - Volume 58, Issue 2, September 2006, Pages 65-69
نویسندگان
Zhi Liu, Qing Hong, Jian-Hong Xu, Wu Jun, Shun-Peng Li,