The international standard ISO/TS 21872-1 to study the occurence of total and pathogenic Vibrio parahaemolyticus and Vibrio cholerae in seafood: ITS improvement by use of a chromogenic medium and PCR

► Occurrence of V. cholerae and V. parahaemolyticus in seafood was studied. ► The ISO reference standard was used in parallel with a PCR method. ► The ISO standard does not allow good recovery of V. parahaemolyticus in oysters. ► Significative occurence of presumably pathogenic V. parahaemolyticus is confirmed. ► Occurrence of trh gene in species other than V. parahaemolyticus is not negligible.