کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4367625 1616662 2011 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel genotyping scheme for Vibrio parahaemolyticus with combined use of large variably-presented gene clusters (LVPCs) and variable-number tandem repeats (VNTRs)
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
A novel genotyping scheme for Vibrio parahaemolyticus with combined use of large variably-presented gene clusters (LVPCs) and variable-number tandem repeats (VNTRs)
چکیده انگلیسی

A total of 18 variably-presented gene clusters (LVPCs) and nine previously characterized variable-number tandem repeats (VNTRs), and all known virulence markers were screened for their frequency and/or copy number in 251 global strains of Vibrio parahaemolyticus using PCR and gel or capillary electrophoresis. A two-step genotyping approach combining the use of LVPCs and VNTRs was established accordingly. The frequency profiles of LVPCs and virulence markers were primarily used to group the strains into six distinct complexes with different potential pathogenicity natures. The strains from each of these complexes were further analyzed with VNTRs to give a much more detailed discrimination of the strains. A genetic fingerprint-like database of a large collection of strains established with this two-stage approach would be very useful for identification, genotyping, origin tracing, and risk estimation of V. parahaemolyticus.


► A two-step genotyping approach was established for Vibrio parahaemolyticus.
► LVPCs and virulence markers were firstly used to group strains into six complexes.
► Strains from each complex were further discriminated with VNTRs.
► This approach could be used for genotyping and origin tracing of V. parahaemolyticus.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Food Microbiology - Volume 149, Issue 2, 15 September 2011, Pages 143–151
نویسندگان
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