کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4367657 | 1616644 | 2012 | 8 صفحه PDF | دانلود رایگان |
Bifidobacterium adolescentis Int57 (Int57) and Propionibacterium freudenreichii subsp. shermanii ATCC 13673 (ATCC 13673) were grown either in coculture or as pure cultures in different media, such as cow's milk, soybean milk, and modified MRS medium. The viable cell counts of bacteria, changes in pH, concentrations of organic acids, and contents of various sugars were analyzed during incubation up to 7 days. In soy milk, the survival of cocultured Int57 was six times higher than the monocultured cells, and ATCC 13673 cocultured with Int57 consumed 69.4% of lactic acid produced by Int57 at the end of fermentation. In cow's milk, coculture with ATCC 13673 increased the growth of Int57 from 24 h until 120 h by approximately tenfold and did not affect the survival of Int57 cells. After 96 h of fermentation of modified MRS, the survival of ATCC 13673 cells cocultured with Int57 increased by 3.2- to 7.4-folds as compared with ATCC 13673 monoculture, whereas the growth of Int57 cells was unaffected. The growth and metabolic patterns of two strains during coculture showed noticeable differences between food grade media and laboratory media. The consumption of stachyose in soy milk during coculture of Int57 with ATCC 13673 was increased by more than twice compared with Int57 monoculture, and completed within 24 h. The combinational use of Bifidobacterium and Propionibacterium could be applied to the development of fermented milk or soy milk products.
► First report on coculture of Propionibacterium and Bifidobacterium in food media.
► P. shermanii enhanced the survival of B.Int57 in soy milk.
► Cocultured fermentation of soy milk reduced the flatulence factor by over 2-folds.
► In cow's milk, P. shermanii increased the number of B.Int57 by approximately 10-folds.
► Promising candidate for starter cultures in soy milk and cow's milk fermentation.
Journal: International Journal of Food Microbiology - Volume 157, Issue 1, 15 June 2012, Pages 65–72