Exploration of phenomena contributing to the diversity of Oenococcus oeni exopolysaccharides

Many food-grade bacteria produce exopolysaccharides (EPS) that may modify the food texture or affect their survival rate during food processing. This is the case of O. oeni, a bacterial species who drives malolactic fermentation in wine. The five strains analyzed in the present study all display both isolated genes dedicated to homopolysaccharide synthesis and gene clusters potentially associated with heteropolysaccharide synthesis. The number of isolated glycosyltransferase gene present and the gene composition of one of the operons change from one strain to the other. The soluble EPS yields and the EPS monomer composition vary depending on the strain and or the medium composition. O. oeni appears as a bacterium able to synthesize both homo and heteropolysaccharides. This unique property has rarely been described. Moreover, the abundance of the genetic determinants associated with EPS metabolism suggests that it is very important for the adaptation of the bacteria to wine.

► O. oeni is the bacterial species which drives malolactic fermentation in wine.
► It can produce both homo and hetero exopolysaccharides (EPS).
► The EPS yield and structure change depending on the strain and growth medium.
► Numerous genetic determinants are found in O. oeni genomes.