Transcription profiling of sparkling wine second fermentation

► We characterized the transcriptome of wine yeast during second fermentation. ► Oxidative metabolism, autophagy, vacuolar and peroxisomal functions are affected. ► Ethanol is the main factor shaping transcription during second fermentation. ► Transcriptome in second fermentation mimics that of primary wine fermentation. ► pH and CO2 are not relevant for the transcription profile in second fermentation.