کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4367983 1616665 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Metabolomes of the psychrotolerant bacterium Listeria monocytogenes 10403S grown at 37 °C and 8 °C
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Metabolomes of the psychrotolerant bacterium Listeria monocytogenes 10403S grown at 37 °C and 8 °C
چکیده انگلیسی

Listeria monocytogenes is a food-borne pathogen with the ability to grow at refrigeration temperatures. Knowledge of the mechanisms involved in low temperature growth is incomplete and here we report the results of a metabolomics investigation of this. The small molecule contents of L. monocytogenes 10403S grown at 37 °C and 8 °C were compared by gas chromatography/mass spectrometry (GC/MS). Over 500 peaks were detected in both 37 °C and 8 °C-grown cells, and 103 were identified. Of the identified metabolites, the concentrations of 56 metabolites were increased (P < 0.05), while the concentrations of 8 metabolites were decreased at low temperature. Metabolites increasing in concentration at 8 °C included amino acids, sugars, organic acids, urea cycle intermediates, polyamines, and different compatible solutes. A principal component analysis (PCA) was used to visualize and compare the matrix containing the data in 6 samples, and this clearly identified the 37 °C and 8 °C metabolomes as different. The results indicated that an increase in solute concentrations in the cytoplasm was associated with low temperature adaptation, which may be a response to chill stress with the effect of lowering the freezing point of intracellular water and decreasing ice crystal formation.

Research highlights
► Molecular contents of L. monocytogenes 10403S grown at 37 °C and 8 °C were compared.
► An increased concentration might be associated with low temperature adaptation.
► Increased concentrations lower the freezing point of intracellular water and decrease ice formation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Food Microbiology - Volume 148, Issue 2, 2 August 2011, Pages 107–114
نویسندگان
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