کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4370087 | 1616764 | 2006 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of environmental stresses on the mean and distribution of individual cell lag times of Escherichia coli O157:H7
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Effect of environmental stresses on the mean and distribution of individual cell lag times of Escherichia coli O157:H7 Effect of environmental stresses on the mean and distribution of individual cell lag times of Escherichia coli O157:H7](/preview/png/4370087.png)
چکیده انگلیسی
The effect of starvation, heat or acid stress on duration of individual cell lag time (Ï) and standard deviation (SD) of Ï was investigated using Escherichia coli O157:H7. Cells were stressed by exposure to acid (pH 3.5), heat (50 °C), or starvation in either glucose-free mineral medium (MOPS), tryptic soy broth (TSB) or Luria broth (LB). Stressed cells were then diluted into wells of a Bioscreen plate to obtain single cells per well. Replicate time to detection (td) values were obtained using the Bioscreen and used to calculate the Ï and SD. Significant (P â¤Â 0.05) increases in Ï over untreated controls were found for the following treatments: 14 days in acid; 2 h of heating; 3 days starvation in MOPS; and 2 days starvation in either TSB or LB. The largest increase in Ï was > 2-fold from 2.5 to 5.6 h observed with the heat treatment. MOPS starvation was more detrimental to the cells than was acid treatment over the same time period. A significant increase in SD was found with 21 days acid treatment, and 2 days starvation in either TSB or LB. No significant increase in SD was found for MOPS starvation or heat treatment. Lognormal, Gamma, ExtremeValue and Weibull distributions were fitted to the Ï data using BestFit. The results suggest that the Lognormal distribution is suitable for fitting Ï data from either stressed or unstressed cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Food Microbiology - Volume 110, Issue 3, 1 August 2006, Pages 278-285
Journal: International Journal of Food Microbiology - Volume 110, Issue 3, 1 August 2006, Pages 278-285
نویسندگان
Yaqin Li, Joseph A. Odumeru, Mansel Griffiths, Robin C. McKellar,