کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4370958 1617000 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Surface binding properties of aged and fresh (recently excreted) Toxoplasma gondii oocysts
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Surface binding properties of aged and fresh (recently excreted) Toxoplasma gondii oocysts
چکیده انگلیسی


• Age of Toxoplasma oocysts affects surface-binding properties.
• Fresh oocysts bound monoclonal antibody, but not old oocysts.
• Old oocysts bound wheat germ agglutinin (WGA), but not fresh oocysts.
• Treating fresh oocysts with detergent or trypsin did not affect WGA binding.
• Fresh oocysts bound WGA after treatment with acidified pepsin.

The surfaces of aged (10 years) and fresh (recently excreted) oocysts of Toxoplasma gondii were investigated using monoclonal antibody (mAb) and lectin-binding assays. Fresh oocysts bound a wall-specific mAb labelled with fluorescein isothiocyanate while aged oocysts did not. In contrast, the walls of aged oocysts bound a lectin (wheat germ agglutinin, WGA), but not the walls of fresh oocysts. Exposure of oocysts to detergent solutions or trypsin did not affect the binding properties of the walls of the oocysts. However, exposure of fresh oocysts to acidified pepsin enabled labelling of the walls with WGA, presumably due to the relevant moieties on the oocyst walls becoming exposed. WGA binding, but not mAb binding, was partially abrogated with periodate exposure. These findings reveal a significant difference in the binding properties of oocyst walls from “aged” and “fresh” oocysts. The results are of relevance when considering technologies for isolating or detecting T. gondii oocysts in environmental samples based on oocyst surface properties, as used for other protozoan parasites. Our results suggest the possibility of developing a WGA-based separation procedure for isolating Toxoplasma oocysts from environmental matrices, in which pepsin pre-treatment would be included to ensure that both fresh and aged oocysts were isolated.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 165, June 2016, Pages 88–94
نویسندگان
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