کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4371237 1302502 2010 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Trypanosoma cruzi: Modulation of HSP70 mRNA stability by untranslated regions during heat shock
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Trypanosoma cruzi: Modulation of HSP70 mRNA stability by untranslated regions during heat shock
چکیده انگلیسی
Gene regulation in trypanosomatids occurs mainly by post-transcriptional mechanisms modulating mRNA stability and translation. We have investigated heat shock protein (HSP) 70 gene regulation in Trypanosoma cruzi, the causal agent of Chagas' disease. The HSP70 mRNA's half-life increases after heat shock, and the stabilization is dependent on protein synthesis. In a cell-free RNA decay assay, a U-rich region in the 3′ untranslated region (UTR) is a target for degradation, which is reduced when in the presence of protein extracts from heat shocked cells. In a transfected reporter gene assay, both the 5′- and 3′-UTRs confer temperature-dependent regulation. Both UTRs must be present to increase mRNA stability at 37 °C, indicating that the 5′- and 3′-UTRs act cooperatively to stabilize HSP70 mRNA during heat shock. We conclude that HSP70 5′- and 3′-UTRs regulate mRNA stability during heat shock in T. cruzi.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 126, Issue 2, October 2010, Pages 245-253
نویسندگان
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