کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4371361 | 1302510 | 2009 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Schistosoma japonicum: A PCR assay for the early detection and evaluation of treatment in a rabbit model
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
انگل شناسی
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![عکس صفحه اول مقاله: Schistosoma japonicum: A PCR assay for the early detection and evaluation of treatment in a rabbit model Schistosoma japonicum: A PCR assay for the early detection and evaluation of treatment in a rabbit model](/preview/png/4371361.png)
چکیده انگلیسی
A specific PCR assay for the detection of Schistosoma japonicum DNA in rabbit fecal and serum samples was developed by amplifying a 230-bp fragment from the sequence information of the clone G55A of the highly repetitive retrotransposon SjR2. The minimum amount of DNA detectable using the PCR assay was 0.8Â pg, and the expected PCR product was amplified when DNA equivalent of 1.1 egg from feces was used as template. In the meantime, serum anti-worm IgG was examined by ELISA. ELISA gave positive results at 4-6 weeks post-infection depending on the cercarial doses. The parasite eggs were detected in feces at 7 weeks post-infection. In contrast, S. japonicum DNA was detected in sera at first week post-infection, and it became negative at 10 weeks post-treatment, whereas the anti-worm IgG was still at high levels at 23 weeks post-treatment. These data demonstrated that the PCR assay established provides a potential tool for the early diagnosis and therapy evaluation for S. japonicum infection in humans.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 121, Issue 2, February 2009, Pages 175-179
Journal: Experimental Parasitology - Volume 121, Issue 2, February 2009, Pages 175-179
نویسندگان
Chao-Ming Xia, Rong Rong, Zheng-Xian Lu, Chang-Jun Shi, Jing Xu, Hui-Qin Zhang, Wei Gong, Wei Luo,