کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4371431 1302519 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Trypanosoma cruzi SHSP16: Characterization of an α-crystallin small heat shock protein
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Trypanosoma cruzi SHSP16: Characterization of an α-crystallin small heat shock protein
چکیده انگلیسی

This report describes the characterization of a member of the α-crystallin small heat shock protein family in a trypanosomatid, which was isolated from the human pathogen Trypanosoma cruzi. One α-crystallin small heat shock protein gene was identified in a database search. The coding region is located in an open reading frame of 429 bp encoding a protein of 142 amino acids. The amino acid sequence was deduced from the isolated gene. The protein has an α-crystallin domain characteristic of the α-crystallin small heat shock proteins and a molecular weight of 15.9 kDa, so the protein was designated SHSP16. Analysis of the nucleotide sequences of four different T. cruzi strains showed two different sequences, which correspond to the two main T. cruzi genetic groups. Gene expression analysis by RT-PCR showed increased transcription of the gene after the parasite was exposed to heat stress. Recombinant SHSP16 showed molecular chaperone activity in vitro, because it inhibited the thermal aggregation of the mitochondrial malate dehydrogenase enzyme.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 123, Issue 2, October 2009, Pages 182–189
نویسندگان
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