کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4371642 | 1302533 | 2010 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification and differentiation of human schistosomes by polymerase chain reaction
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
انگل شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Identification and differentiation of human schistosomes by polymerase chain reaction Identification and differentiation of human schistosomes by polymerase chain reaction](/preview/png/4371642.png)
چکیده انگلیسی
Recent increasing number of travelers, immigrants and foreign workers from schistosomiasis endemic area has thus resulted in the importation of schistosomiasis to non-endemic countries. To avoid ova-induced pathogenicity, sensitive and specific diagnostic means at an early stage of infection are therefore crucial. In this study, we developed polymerase chain reaction (PCR) primers specific for human schistosome species. The PCR products were obtained in a species-specific manner (479Â bp, Schistosoma mansoni; 365Â bp, S. haematobium; 614Â bp, S. japonicum; 303Â bp, S. mekongi) and were detectable from 0.01Â pg of total worm DNA (S. haematobium, S. japonicum, S. mekongi). The primer sets were also available for multiplex use. Although some difficulties were experienced in amplifying the parasite DNA from the infected animals, schistosome DNA could be detected from one day post infection. The PCR method described herein will therefore be beneficial to detect human schistosomiasis, after some improvements in this method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 124, Issue 3, March 2010, Pages 325-329
Journal: Experimental Parasitology - Volume 124, Issue 3, March 2010, Pages 325-329
نویسندگان
Naoko Kato-Hayashi, Masashi Kirinoki, Yukio Iwamura, Tamotsu Kanazawa, Viroj Kitikoon, Hajime Matsuda, Yuichi Chigusa,