کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4371781 | 1302540 | 2008 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Trypanosoma evansi: Molecular homogeneity as inferred by phenetical analysis of ribosomal internal transcribed spacers DNA of an eclectic parasite
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کلمات کلیدی
ITSRDNARAPDRFLPTrypanosoma evansiNTSkDNAarbitrary primersRibosomal DNA - DNA ریبوزومیKinetoplast DNA - DNA کینتوپلاستdNTPS - DNTPSInternal transcribed spacer - Spacer رونویسی داخلیdeoxyribonucleotide triphosphates - تری فسفاتهای deoxyribonucleotidepolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازrestriction fragment length polymorphism - پلی مورفیسم طول قطعه قطعه
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
انگل شناسی
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چکیده انگلیسی
The protozoan Trypanosoma evansi is described as presenting high morphological and genetic similarities among the isolates despite its biological heterogeneity and wide geographical distribution. PCR amplification of the internal transcribed spacers of the ribosomal gene in combination with the coding region of the 5.8S ribosomal subunit further submitted to restriction enzymes digestion were carried out in DNAs extracted from 41 T. evansi strains isolated from horses, dogs, coatis and capybaras from two distinct regions of the Brazilian Pantanal. We also used one T. evansi isolate from Africa, one from Asia and one isolate of T. b. brucei from Africa. Analysis of the RFLP profiles yielded a unique “riboprinting” that does not vary intraspecifically. These results provide insights on the ribosomal gene organization of T. evansi and showed that ITS analysis by RFLP show high genetic similarity of this locus among isolates of this protozoan parasite.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 118, Issue 3, March 2008, Pages 402-407
Journal: Experimental Parasitology - Volume 118, Issue 3, March 2008, Pages 402-407
نویسندگان
Aneska Norek de Oliveira Lima, Simone da Silva Santos, Heitor Miraglia Herrera, Carla Gama, Elisa Cupolillo, Ana Maria Jansen, Octávio Fernandes,