کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4372154 | 1302573 | 2006 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Entamoeba histolytica: Inflammatory process during amoebic liver abscess formation involves cyclooxygenase-2 expression in macrophages and trophozoites Entamoeba histolytica: Inflammatory process during amoebic liver abscess formation involves cyclooxygenase-2 expression in macrophages and trophozoites](/preview/png/4372154.png)
It has been demonstrated that expression of cyclooxygenase-2 (COX-2) isoform is induced by Entamoeba histolytica in macrophages and polymorphonuclear cells during amoebic liver abscess (ALA) formation in hamsters. Trophozoites present in the lesion were also positive for COX-2 signal. However, no cross reactivity of the anti-COX-2 antibody with protein extract of cultivated trophozoites was found. To clarify if trophozoites are involved in PGE2 production during ALA development, COX-2 expression was detected by in situ hybridization and RT-PCR in liver tissue from intrahepatically infected hamsters. COX-2 mRNA was in polymorphonuclear cells since 4 h postinfection, and subsequently, local macrophages expressed COX-2 mRNA in a similar way. Additionally, a positive signal for COX-2 mRNA expression was detected in E. histolytica trophozoites, suggesting that, in vivo, parasite COX expression may be an important mechanism to promote inflammation.
Journal: Experimental Parasitology - Volume 114, Issue 3, November 2006, Pages 154–159