Preparation of vanillin by bioconversion in a silicon rubber membrane bioreactor

In this study, the bioconversion of clove oil into vanillin using soybean lipoxygenase (SBLOX) as biocatalyst was investigated in a silicon rubber membrane bioreactor (SRMBR) and shaking flasks. High performance liquid chromatography (HPLC) analysis indicated that the vanillin concentration was 8.14 mg/L after 36 h of conversion in a shaking flask. It reached up to 121.53 mg/L in the receiving solution after 36 h of conversion in the SRMBR. The conversion rate of clove oil was 0.033% in the shaking flask. It was 1.01% in the SRMBR. The peak area ratio of vanillin in the receiving solution of the SRMBR was 70.08%. By adding activated carbon into the conversion broth of the SRMBR, the vanillin concentration in the receiving solution reached 140.27 mg/L, the conversion rate of clove oil increased to 1.14%, and the peak area ratio of vanillin in the receiving solution reached 93.53%.