کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4406484 1618687 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Recombinant expression and purification of human placental growth factor 1 and specific camel heavy chain polyclonal antibody preparation
ترجمه فارسی عنوان
بیان واکنش و تصفیه فاکتور رشد جسمی انسانی 1 و تهیه آنتی بادی پلی کلنال زنجیره سنگین شتر خاص
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بوم شناسی
چکیده انگلیسی

Placental growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family. Unlike VEGF, PlGF is dispensable for normal cell development as well as playing various roles in pathological angiogenesis which occurs in tissue ischemia, inflammation, and malignancy. The PlGF-1 has been considered as a potential candidate for the diagnosis and targeting of pathological angiogenesis. Camelidae serum contains an important fraction of functional antibodies, called heavy-chain antibodies (HcAbs) that are naturally devoid of light chains. Camelid HcAbs recognize their cognate antigens by a single variable-domain, referred to as VHH or Nanobody.Here, we describe the expression and purification of recombinant human PlGF-1 (rhPlGF-1). This protein was subsequently used for the preparation of camel heavy chain polyclonal antibody against rhPlGF-1.The recombinant expression plasmid pET-26b-hPlGF-1 was introduced into Escherichia coli BL21 cells to express the rhPlGF-1 protein. Purified rhPlGF-1 was used to immunize camel, the specific reactivity of HcAb was determined with ELISA and western blot. Western blot analysis indicated that the antiserum specifically reacted to the recombinant protein. The rhPlGF-1 protein and its antibody may be used for the development of detection assays needed for clinical research.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Saudi Journal of Biological Sciences - Volume 21, Issue 1, January 2014, Pages 35–39
نویسندگان
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