Synthesis of silver nanoparticle and its application

• Silver nanoparticles have been synthesized by wet chemical technique, green synthesis and microbial methods.
• Synthesis of nanoparticles by green synthesis yields high nanoparticles.
• Synthesized nanoparticles were characterized by UV, SEM and FTIR analyses.
• Its cytotoxic effect was studied in cancerous cell line and normal cell line.

In this work, silver nanoparticles have been synthesized by wet chemical technique, green synthesis and microbial methods. Silver nitrate (10−3 M) was used with aqueous extract to produce silver nanoparticles. From the results it was observed that the yield of nanoparticles was high in green synthesis. The size of the silver nanoparticles was determined from Scanning Electron Microscope analysis (SEM). Fourier Transform Infrared spectroscopy (FTIR) was carried out to determine the presence of biomolecules in them. Its cytotoxic effect was studied in cancerous cell line and normal cell line. MTT assay was done to test its optimal concentration and efficacy which gives valuable information for the use of silver nanoparticles for future cancer therapy.