کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4419469 1618936 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Toxicological and chemical investigation of untreated municipal wastewater: Fraction- and species-specific toxicity
ترجمه فارسی عنوان
بررسی سمی و شیمیایی پساب های غیر بهداشتی شهری: سمیت اختصاصی فراورده و گونه
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
چکیده انگلیسی


• Polar fraction is the main carrier of toxic effect of municipal wastewater.
• PLHC-1, H4IIE and HepG2 cells exhibit different responses in toxicity assays.
• Human derived cells should be used in toxicity assessment of chemical mixtures.
• The untreated wastewater mainly contains hydrocarbons, alkylphenols, plasticizers.

Absence of a municipal wastewater (WW) treatment plant results in the untreated WW discharge into the recipient. The present study investigated toxic effects and chemical composition of water extracts and fractions from untreated WW and recipient Danube River (DR). Samples were prepared by solid-phase extraction and silica gel fractionation and screened for EROD activity and cytotoxicity using aquatic models, comprising of fish liver cells (PLHC-1) and a model of the early development of zebrafish embryos, while rat (H4IIE) and human (HepG2) hepatoma cells served as mammalian models. Polar fraction caused cytotoxicity and increased the EROD activity in PLHC-1 cells, and increased mortality and developmental abnormalities in developing zebrafish embryos. In H4IIE, polar fraction induced inhibition of cell growth and increased EROD activity, whereas HepG2 exerted low or no response to the exposure. Non-polar and medium-polar fractions were ineffective. Tentative identification by GC/MS showed that WW is characterized by the hydrocarbons, alkylphenols, plasticizers, and a certain number of benzene derivatives and organic acids. In DR, smaller number of organic compounds was identified and toxicity was less pronounced than in WW treatments. The present study revealed the potent toxic effect of polar fraction of untreated WW, with biological responses varying in sensitivity across organisms. Obtained results confirmed that fraction- and species-specific toxicity should be considered when assessing health risk of environmental pollution.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ecotoxicology and Environmental Safety - Volume 127, May 2016, Pages 153–162
نویسندگان
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