کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4419904 1618953 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The change in heat shock protein expression in avermectin induced neurotoxicity of the pigeon (Columba livia) both in vivo and in vitro
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
The change in heat shock protein expression in avermectin induced neurotoxicity of the pigeon (Columba livia) both in vivo and in vitro
چکیده انگلیسی


• The expression of Hsps exposed to AVM was investigated for pigeon neurons.
• The mRNA and protein levels of Hsps were increased in vivo and in vitro.
• AVM exposure can induce a protective stress response in pigeon brain.

The expression of heat shock proteins (Hsps) commonly increases to provide neuroprotection when brain tissues are under stress conditions. Residues of avermectins (AVMs) have neurotoxic effects on a number of non-target organisms. The aim of this study was to investigate the effects of AVM exposure on the expression levels of Hsp 60, Hsp 70 and Hsp 90 for pigeon (Columba livia) neurons both in vivo and in vitro. The results showed that in general, the mRNA and protein levels of Hsps were increased in treated groups relative to control groups after AVM exposure for 30 d, 60 d and 90 d in the cerebrum, cerebellum and optic lobe in vivo. However, AVM exposure had no significant effects on the transcription expression of Hsps for 90 d in the optic lobe and decreased the translation expression of Hsps significantly for 90 d in the optic lobe. In vitro, the LC50 of avermectin for King pigeon neurons is between 15 μg L−1 and 20 μg L−1. Following AVM (2.5–20 μg L−1) exposure, the mRNA expression of the 3 Hsps was up-regulated to different degrees. Compared with the control groups, a significant decrease, a remarkable increase and a non-significant change was found in the protein expression of Hsp 60, Hsp 70 and Hsp 90 separately following AVM (2.5–20 μg L−1) exposure. Based on these results, we conclude that AVM exposure can induce a protective stress response in pigeons by means of promoting the mRNA and protein expression of Hsps under in vivo and in vitro conditions, thus easing the neurotoxic effects of AVM to some extent.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ecotoxicology and Environmental Safety - Volume 110, December 2014, Pages 95–102
نویسندگان
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