Toxicity on the luminescent bacterium Vibrio fischeri (Beijerinck). II: Response to complex mixtures of heterogeneous chemicals at low levels of individual components

The toxicity of eight complex mixtures of chemicals with different chemical structures and toxicological modes of action (narcotics, polar narcotics, herbicides, insecticides, fungicides) was tested on the luminescent bacterium Vibrio fischeri. There were maximum 84 individual chemicals in the mixtures. Suitable statistical approaches were applied for the comparison between experimental results and theoretical predictions. The results demonstrated that the two models of Concentration Addition (CA) and Independent Action (IA) are suitable to explain the effect of the mixtures.Even extremely lower concentrations of individual chemicals contributed to the effect of the mixtures. Synergistic effects were not observed in any of the tested mixtures.In particular, the CA approach well predicted the effects of six out of eight mixtures and slightly overestimated the effects of the remaining two mixtures. Therefore, the CA model can be proposed as a pragmatic and adequately protective approach for regulatory purposes.

► Complex mixtures have been tested on luminescent bacteria (Microtox® test).
► CA and IA models are suitable to predict mixture effects.
► The coherence with theoretical models have been statistically tested.
► Even extremely lower concentrations, far below NOEC, contribute to the combined effect.
► The CA approach can be assumed as a protective choice for regulatory purposes.