Using the enzymatic and non-enzymatic antioxidant defense system of the land snail Eobania vermiculata as biomarkers of terrestrial heavy metal pollution

The present study aimed to investigate the efficacy of antioxidant defense system of the land snail Eobania vermiculata as biomarkers for terrestrial heavy metal pollution. For this reason, a set of biomarkers was performed on land snails E. vermiculata collected from polluted and non-polluted areas in the field. The biomarkers used were lactate dehydrogenase activity, level of lipid peroxidation, activities of catalase, glutathione peroxidase, glutathione-S-transferase, gamma-glutamyl transferase and content of glutathione, metalothionines, total proteins and total lipid in the digestive gland tissue. The correlation between the bioaccumulation of heavy metal in gland tissue and all the biomarkers in the digestive gland was determined. The results revealed appreciable alterations in the biomarker values in field, accompanied by significant correlations among the biomarkers. Therefore, this study suggests E. vermiculata is a suitable sentinel organism and the selected biomarkers show efficacy for terrestrial heavy metal biomonitoring.

► Significant changes were noted in the values of the applied biomarkers.
► A package of oxidative stress biomarkers is supported to be an efficient tool for biomonitoring studies.
► The land snail Eobania vermiculata is proposed to be a good sentinel organism in terrestrial metal pollution studies.