کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4420986 1618984 2012 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of genes associated with adaptation to NaCl toxicity in perennial ryegrass (Lolium perenne L.)
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
Identification of genes associated with adaptation to NaCl toxicity in perennial ryegrass (Lolium perenne L.)
چکیده انگلیسی

Perennial ryegrass (Lolium perenne L.) is a popular turfgrass species. To understand the molecular mechanisms of salinity tolerance, a suppression subtractive cDNA library was constructed for a salinity-tolerant ryegrass accession, with NaCl-treated (255 mM) plants as the tester. Differentially expressed cDNA fragments were cloned and screened. BLAST search revealed that 268 clones exhibited significant homologies to known genes. These genes could be categorized into 11 different functional groups, including metabolism, energy transfer, detoxification, compatible solute, cellular transport, transcription, signal transduction, etc. The salinity-regulated expression of selected genes was confirmed by RT-PCR analysis. The results suggested that these putatively salinity up-regulated genes may play a vital role in the salinity tolerance of perennial ryegrass. They can be used as candidate genes for creating stress-tolerant grasses and for understanding molecular mechanisms of plant adaptation to salinity stress.


► A suppression subtractive cDNA library was constructed for a salinity-tolerant perennial ryegrass accession.
► 268 salinity up-regulated cDNA clones were obtained, which could be categorized into 11 different functional groups.
► These salinity up-regulated genes may play a vital role in the salinity tolerance of perennial ryegrass.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ecotoxicology and Environmental Safety - Volume 79, 1 May 2012, Pages 153–162
نویسندگان
, , ,