An assessment of the genotoxic effects of landfill leachates using bacterial and plant tests

Two bacterial tests (the Ames test and the umu-test) and the Allium test were used to assess the genotoxicity of aqueous leachates from municipal solid waste landfill sites in Southern Poland. A comparison of the sensitivity of the applied tests was performed. None of the tested samples revealed genotoxic activity in the umu-test and thus did not appear to be sensitive enough for evaluations of leachates. Two out of 22 leachates were described as genotoxic in the Ames test and 3 out of 6 leachates in the Allium test. All of the analyzed leachates samples affected cell divisions. Two of the tests applied, the Allium and Ames test, revealed the high genotoxicity of leachate 4.Among the bioassays used in these studies, the Allium test proved to be more sensitive than bacterial tests for the investigation of leachate toxicity. The results suggest that the Allium cytogenetic bioassay is efficient and simple for genotoxicity studies of leachates. The potential correlations between the chemical characteristics and genotoxic effects is discussed. The biological effects of selected appeared to be related to chemical parameters. Leachates have a genotoxic potential and pose a risk to human health and the environment. A combination of biotests and chemical analyses is the best approach for the assessment of the risk or impact of leachates.

► We used Ames test, umu-test and Allium test to assess the genotoxicity of leachates from municipal waste landfills in Poland.
► The comparison of the sensitivity of the applied tests was performed.
► umu-test is not sensitive enough for leachates evaluations, Allium test was more sensitive than bacterial tests.
► Two out of 22 leachates were described as genotoxic in Ames test, and 3 out of 6 leachates in Allium test.
► The potential correlations between the chemical characteristic and genotoxic effects were discussed.