کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4422911 | 1619077 | 2012 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Mechanistic insights into nanotoxicity determined by synchrotron radiation-based Fourier-transform infrared imaging and multivariate analysis Mechanistic insights into nanotoxicity determined by synchrotron radiation-based Fourier-transform infrared imaging and multivariate analysis](/preview/png/4422911.png)
Our ability to identify the mechanisms by which carbon-based nanomaterials (CBNs) exert toxicity in cells is constrained by the lack of standardized methodologies to assay endpoint effects. Herein we describe a method of mechanistically identifying the effects of various CBN types in both prokaryotic and eukaryotic cells using multi-beam synchrotron radiation-based Fourier-transform infrared imaging (SR-FTIRI) at diffraction-limited resolution. This technique overcomes many of the inherent difficulties of assaying nanotoxicity and demonstrates exceptional sensitivity in identifying the effects of CBNs in cells at environmentally-relevant concentrations. We identify key mechanisms of nanotoxicity as the alteration of Amide and lipid biomolecules, but propose more specific bioactivity of CBNs occurs as a result of specific interactions between CBN structural conformation and cellular characteristics.
Figure optionsDownload as PowerPoint slideHighlights
► Gram-negative bacteria are more susceptible to nanotoxicity than Gram-positive.
► Amide and lipid biomolecule alterations are consistent mechanisms of nanotoxicity.
► Specific bioactivity is a function of cell and CBN type combinations.
► IR spectroscopy with multivariate analysis is a robust method of assaying nanotoxicity.
Journal: Environment International - Volume 50, 1 December 2012, Pages 56–65