کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4424832 1309086 2011 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Aporrectodea caliginosa, a suitable earthworm species for field based genotoxicity assessment?
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
Aporrectodea caliginosa, a suitable earthworm species for field based genotoxicity assessment?
چکیده انگلیسی

There is a growing interest for the application of biomakers to field-collected earthworms. Therefore we have evaluated the usability of native populations of endogeic, widely distributed earthworm Aporrectodea caliginosa in the assessment of soil genotoxicity using the Comet assay. Validation of the Comet assay on earthworm coelomocytes has been established using commercially available Eisenia fetida exposed to copper, cadmium, and pentachlorophenol, along with A. caliginosa exposed to copper in a filter paper contact test. Neutral red retention time (NRRT) assay was conducted on copper exposed and field-collected earthworms. Significant DNA and lysosomal damage was measured using Comet and NRRT assays in native populations of A. caliginosa sampled from the polluted soils in the urban area in comparison to the earthworms from the reference site. The results of this study confirm the employment of A. caliginosa as a suitable species for the in situ soil toxicity and genotoxicity field surveys.

Research highlights
► Native A. caliginosa has shown significant biological effect measured by the Comet and NRRT assays.
► The Comet assay on A. caliginosa and E. fetida has shown to be of similar sensitivity as the NRRT assay.
► A. caliginosa is a suitable species for the in situ soil toxicity and genotoxicity field surveys.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental Pollution - Volume 159, Issue 4, April 2011, Pages 841–849
نویسندگان
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