The Nile monitor (Varanus niloticus; Squamata: Varanidae) as a sentinel species for lead and cadmium contamination in sub-Saharan wetlands

Wetland pollution is a matter of concern in sub-Saharan Africa. Though regularly exploited, the Nile monitor (Varanus niloticus), a large amphibious lizard, is not threatened. This work aims at assessing the value of this varanid as a sentinel species in surveys of environmental contamination by metals. Lead and cadmium quantifications were performed by graphite furnace-atomic absorption spectrophotometry in bone, intestine, kidney, liver and muscle in 71 monitors from three unevenly polluted sites in Mali and Niger, plus a reference site. The effects of sex, size and fat reserves as well as factors related to the sampling strategy (tissue sampled, sampling site) were studied with a mixed linear model. Metal contamination is moderate at the four sites but clear differences nevertheless occur. Lead levels are generally maximal in bone, with a gender-independent median value 320 ng.g−1. Median cadmium concentrations never exceed 70.2 ng.g−1 in females (kidney) and 57.5 ng.g−1 in males (intestine). Such levels should have no detrimental effects on the monitors. Lead and cadmium levels in muscles are generally below 200 and 20 ng.g−1, respectively, and should provoke no health hazard to occasional consumers of monitor meat. Metal organotropisms are consistent with those observed in other studies about Squamates: for lead: bone > [kidney, intestine, liver] > muscle in males and [bone, kidney] > [intestine, liver] > muscle in females; for cadmium: [liver, intestine, kidney] > [bone, muscle] for both genders. Females are more contaminated, especially in their kidneys. In this tissue, median values in ng.g−1 are 129.7 and 344.0 for lead and 43.0 and 70.2 for cadmium, for males and females, respectively. Nile monitors can reveal subtle differences in local pollution by metals; moreover, the spatial resolution of the pollution indication that they give seems to be very sharp. The practical relevance of this new tool is thus validated.

► V. niloticus as an indicator of metallic pollution shows a sharp spatial resolution.
► Metals organotropisms in monitors comply with experimental results in other lizards.
► Females are more heavily contaminated than males, especially in kidneys.
► Lead concentration in bone is not influenced by size.