Aerosolisation of Escherichia coli and associated endotoxin using an improved bubbling bioaerosol generator

► Performances of an improved bubbling bioaerosol generator have been characterised. ► Controlled airborne endotoxin can be generated from gram-negative bacteria suspension. ► Bubbling generator leads to controllable reproduction of bioaerosol concentrations. ► Stability/reproducibility can be improved when using a real-time indicator.