کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4453672 1620810 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Exploration of the key functional proteins from an efficient cellulolytic microbial consortium using dilution-to-extinction approach
ترجمه فارسی عنوان
اکتشاف پروتئین های عملکردی مهم از یک کنسرسیوم میکروبی سلولولیتیک کارآمد با استفاده از رویکرد رقیق شدن تا انقراض
کلمات کلیدی
کنسرسیوم میکروبی، سلولولیتیک، پروتئین اتصال سلولز، روش انحلال تا انقراض
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست علوم زیست محیطی (عمومی)
چکیده انگلیسی

In the present study, the cellulose binding proteins (CBPs) secreted by a putative cellulolytic microbial consortium were isolated and purified by affinity digestion. The purified CBPs were subsequently separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). Using mass spectrometric analyses, eight CBPs were identified and annotated to be similar to known proteins secreted by Clostridium clariflavum DSM 19732 and Paenibacillus sp. W-61. In addition, in combination with dilution-to-extinction approach and zymogram analysis technique, CBPs 6 (97 kDa) and 12 (52 kDa) were confirmed to be the key functional proteins that influence cellulolytic activities. Moreover, structural domain analyses and enzymatic activity detection indicated that CBPs 6 and 12 contained glycoside hydrolase families (GH) 9 and 48 catalytic modules, which both revealed endoglucandase and xylanase activities. It was suggested that the coexistence of GH9 and GH48 catalytic domains present in these two proteins could synergistically promote the efficient degradation of cellulose.

The efficient cellulolytic ability of a previously constructed microbial consortium was found to be correlated to the cellulose binding proteins (CBPs 6 and 12, which containing glycoside hydrolase families 9 and 48 catalytic modules respectively), and these two proteins can synergistically promote the efficient degradation of cellulose.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Environmental Sciences - Volume 43, May 2016, Pages 199–207
نویسندگان
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