Production of bacterial α-amylase by B. amyloliquefaciens under solid substrate fermentation

Production of α-amylase by Bacillus amyloliquefaciens, under solid substrate fermentation (SSF) was investigated in shaken-culture. The maximum α-amylase activity was obtained under the following optimized conditions: corn gluten meal (CGM) 30 g/l, yeast extract (YE) 10 g/l, agitation rate 150 rpm and fermentation temperature 33 °C. The results showed that α-amylase production in a medium with CGM was five times higher than that in the medium contained starch and other components. The temperature of fermentation was found to be most crucial factor in α-amylase production.