کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4493453 1623666 2014 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ameliorative effect of vitamin C against 5-fuorouracil-induced hepatotoxicity in mice: A light and electron microscope study
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Ameliorative effect of vitamin C against 5-fuorouracil-induced hepatotoxicity in mice: A light and electron microscope study
چکیده انگلیسی

5-Fluorouracil is one of the most widely used chemotherapeutic agents in case of hepatic neoplasms. The object of this study was to determine the effectiveness of vitamin C in alleviating 5-fluorouracil-induced hepatotoxicity in male mice. Thirty male albino mice were divided equally into 3 groups, each of 10 animals; group 1, mice received normal saline solution (control group); group 2, mice received 5-fluorouracil 80 mg/kg b.wt./day intraperitoneally for 4 weeks (5-fluorouracil group); group 3, mice received 5-fluorouracil 80 mg/kg b.wt./day for 4 weeks with daily injection of vitamin C (12 mg/kg b.wt./day) for 4 weeks. Animals of all groups were sacrificed and tissue samples from the liver were taken and processed for both light and electron microscopical examination. Light microscopic observations revealed that administration of 5-fluorouracil causes variable signs of hepatotoxicity which are represented by focal areas of liver cell necrosis with distortion of normal hepatic architecture; the hepatocytes showed vacuolated cytoplasm and pyknotic nuclei together with inflammatory cell infiltration. Dilated, congested hepatic sinusoids with active kupffer cells were also seen. Ultrastructure examination confirmed the light microscopic findings and demonstrated vacuolated hepatocytes cytoplasm, dilated endoplasmic reticulum, increased lysosomes, electron-dense mitochondria and pyknotic nuclei. Treatment with vitamin C with 5-fluorouracil attenuated 5-fluorouracil-induced hepatotoxicity and reverted the abnormal structural changes to near normal. In conclusion, these results suggest that vitamin C has a protective potential in ameliorating 5-fluorouracil-induced hepatotoxicity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Basic & Applied Zoology - Volume 67, Issue 4, August 2014, Pages 109–118
نویسندگان
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