کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4498990 | 1319009 | 2007 | 12 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Mechanisms of calcium sequestration during facilitation at active zones of an amphibian neuromuscular junction Mechanisms of calcium sequestration during facilitation at active zones of an amphibian neuromuscular junction](/preview/png/4498990.png)
The calcium transients (Δ[Ca2+]i)(Δ[Ca2+]i) at active zones of amphibian (Bufo marinus ) motor-nerve terminals that accompany impulses, visualized using a low-affinity calcium indicator injected into the terminal, are described and the pathways of subsequent sequestration of the residual calcium determined, allowing development of a quantitative model of the sequestering processes. Blocking the endoplasmic reticulum calcium pump with thapsigargin did not affect Δ[Ca2+]iΔ[Ca2+]i for a single impulse but increased its amplitude during short trains. Blocking the uptake of calcium by mitochondria with CCCP had little effect on Δ[Ca2+]iΔ[Ca2+]i of a single impulse but greatly increased its amplitude during short trains. This present compartmental model is compatible with our previous Monte Carlo diffusion model of Ca2+Ca2+ sequestration during facilitation [Bennett, M.R., Farnell, L., Gibson, W.G., 2004. The facilitated probability of quantal secretion within an array of calcium channels of an active zone at the amphibian neuromuscular junction. Biophys. J. 86(5), 2674–2690], with the single plasmalemma pump in that model now replaced by separate pumps for the plasmalemma and endoplasmic reticulum, as well as the introduction of a mitochondrial uniporter.
Journal: Journal of Theoretical Biology - Volume 247, Issue 2, 21 July 2007, Pages 230–241