کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4501791 | 1320503 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Prediction and Expression Analysis of miRNAs Associated with Heat Stress in Oryza sativa
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Prediction and Expression Analysis of miRNAs Associated with Heat Stress in Oryza sativa Prediction and Expression Analysis of miRNAs Associated with Heat Stress in Oryza sativa](/preview/png/4501791.png)
چکیده انگلیسی
Computational prediction of potential microRNAs (miRNAs) and their target genes was performed to identify the miRNAs and genes associated with temperature response in rice. The data of temperature-responsive miRNAs of Arabidopsis, and miRNAs and the whole genome data of rice were used to predict potential miRNAs in Oryza sativa involved in temperature response. A total of 55 miRNAs were common in both the species, and 27 miRNAs were predicted at the first time in rice. Target genes were searched for these 27 miRNAs in rice genome following stringent criteria. Real time PCR based on expression analysis of nine miRNAs showed that majority of the miRNAs were down regulated under heat stress for rice cultivar Nagina 22. Furthermore, miR169, miR1884 and miR160 showed differential expression in root and shoot tissues of rice. Identification and expression studies of miRNAs during heat stress will advance the understanding of gene regulation under stress in rice.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Rice Science - Volume 21, Issue 1, January 2014, Pages 3-12
Journal: Rice Science - Volume 21, Issue 1, January 2014, Pages 3-12
نویسندگان
B. SAILAJA, S.R. VOLETI, D. SUBRAHMANYAM, N. SARLA, V. VISHNU PRASANTH, V.P. BHADANA, S.K. MANGRAUTHIA,