کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4507400 | 1321353 | 2010 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Simultaneous elimination of Cucumber mosaic virus and Cymbidium mosaic virus infecting Vanilla planifolia through meristem culture Simultaneous elimination of Cucumber mosaic virus and Cymbidium mosaic virus infecting Vanilla planifolia through meristem culture](/preview/png/4507400.png)
Vanilla (Vanilla planifolia G. Jackson), a perennial fleshy vine infected by Cucumber mosaic virus (CMV) and Cymbidium mosaic virus (CymMV) was freed from infection through meristem culture. Apical meristem measuring 0.1–0.25 mm were isolated and cultured in Murashige and Skoog (MS) medium supplemented with 0.45 μM thidiazuron for 40–45 days to initiate the growth. Following enlargement of meristem, it was transferred to MS medium supplemented with 4.43 μM 6-benzyl aminopurine (BAP) and 2.68 μM α-naphthalene acetic acid (NAA) for regeneration. The regenerated plantlets were hardened in insect-free glasshouse and confirmed for the elimination of viruses using reverse transcription PCR (RT-PCR) with virus specific primer pairs. The frequency of CMV elimination was 79.4% while that of CymMV was 82.4% when tested individually. We obtained a frequency of 75% simultaneous elimination of both viruses. This is the first report of meristem culture and virus elimination in vanilla.
Journal: Crop Protection - Volume 29, Issue 10, October 2010, Pages 1214–1217