Analysis of linoleic acid hydroperoxides generated by biomimetic and enzymatic systems through an integrated methodology

An integrated methodology for the identification and quantification of linoleic acid hydroperoxides (HPODEs) mixtures, obtained by the Fenton's reaction and by enzymatic oxidation, is reported. Unambiguous identification of the HPODEs formed (13-hydroperoxy-(9Z,11E)-octadecadienoic acid, 13-hydroperoxy-(9E,11E)-octadecadienoic acid and 9-hydroperoxyoctadecadienoic acid) was performed by HPLC–MS/MS analysis, while quantification was carried out by HPLC–UV using an external calibration, based on the collection of chromatographic peaks, and measuring of total hydroperoxides by ferrous oxidation-xylenol orange (FOX) method.

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► Oxidation of unsaturated fatty acids contributes to their valorization.
► Fatty acids hydroperoxides are high value products at industrial level.
► New pathways to obtain fatty acids hydroperoxides are being studied.
► An integrated methodology for their analysis and quantification is developed.
► This methodology is based on HPLC–MS/MS, HPLC–UV and FOX assay.