Promotive effects of long-term fertilization on growth of tissue culture-derived Hypericum polyanthemum plants during acclimatization

The effect of long-term fertilization supply on biomass yield and secondary metabolites accumulation was assessed in vegetative and reproductive parts of acclimatized field grown plants of Hypericum polyanthemum, an endemic species of southern Brazil. Fertilization caused positive responses in plant growth with biomass increment of vegetative and reproductive parts, proportional to the MS solution concentration (25%, 50% and 100%), whereas the chemical analyses indicated that the supply of fertilization solution did not alter the concentration of 6-isobutyryl-5,7-dimethoxy-2,2-dimethyl-benzopyran (HP1), 7-hydroxy-6-isobutyryl-5-methoxy-2,2-dimethyl-benzopyran (HP2), uliginosin B as well as total phenolic compounds (TPCs), and increased the accumulation of 5-hydroxy-6-isobutyryl-7-methoxy-2,2-dimethyl-benzopyran (HP3) in all fertilization regimes.