Lethal effects of CO2-modified atmospheres for the control of three Bruchidae species

• CO2-MAs are effective in controlling Callosobruchus maculatus, Acanthoscelides obtectus, Zabrotes subfasciatus.
• Tolerance to CO2 varied among the three bruchid species.
• Pupae and eggs were the most tolerant stages, while adults were less tolerant.
• The susceptibility of eggs varied according to their phase of development.
• The susceptibility of eggs was also different in the three species tested.

Bruchid beetles are important pests of field and stored legumes, causing great economic losses. The main pest species are Callosobruchus maculatus, Acanthoscelides obtectus and Zabrotes subfasciatus. Modified atmospheres (MAs) with high carbon dioxide (CO2) content are environmentally friendly pest control methods for stored products. They are effective in controlling a wide range of species and can be used to treat different food products without leading to an accumulation of toxic residues. The present study aimed to establish the efficacy of using MAs with high CO2 to control all developmental stages of these three bruchid pest species. Three high CO2 MAs (50%, 70% and 90%) were tested at 28 °C. In general, pupae and/or eggs were the most tolerant stages to hypercarbia, while adults were less tolerant. The susceptibility of eggs varied greatly according to their phase of development and the pattern was different in the three species tested. Tolerance of other stages to CO2 also varied among the three bruchid species. To achieve total mortality of Z. subfasciatus, a maximum of 9–11 days were needed, depending on CO2 concentration, to kill the eggs. This stage and the pupae were the most tolerant stages. However, in A. obtectus and C. maculatus, only pupae were the most tolerant and required 9 days to be killed, their eggs being more sensitive to CO2.

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