کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4518280 1625000 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Influence of hot water treatment on brown rot of peach and rapid fruit response to heat stress
ترجمه فارسی عنوان
تأثیر استفاده از آب گرم در پودر قهوه پاسخ هویج و سریع میوه به استرس گرما
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
چکیده انگلیسی


• A curative activity of heat (HT) was found on peach inoculated with M. laxa 48 h before treatment.
• Cell wall genes involved in ripening (β-GAL, PL, PG, PME) showed a general decrease in expression in HT fruit as compared to the control.
• ROS scavenging genes and PAL, CHI, HSP70 increased their expression levels in HT samples as compared untreated fruit.
• Each analyzed gene proved to be differentially expressed following heat treatment.

Recent results on hot water as an alternative treatment open a new perspective in disease incidence reduction. In the present work peach fruit were wounded, inoculated with conidia of Monilinia laxa and 15 min, 3, 6, 12, 24 and 48 h after inoculation treated by dipping in hot water (HT) at 60 °C for 20 s. The effect of heat treatment on some cell wall genes involved in ripening such as β-galactosidase (β-GAL), pectin lyase (PL), polygalacturonase (PG) and pectin methyl esterase (PME), was analyzed by qRT-PCR. The expression levels of defense related genes, phenylalanine ammonia lyase (PAL) and chitinase (CHI), heat stress-related genes such as heat shock proteins 70 and 90 (HSP70, HSP90), and reactive oxygen species (ROS) scavenging genes were also evaluated by qRT-PCR. A 100% disease incidence reduction, as compared to untreated fruit, was obtained by treating 6 and 12 h after inoculation. Moreover, brown rot was inhibited by 85.7% when fruit were heat-treated 48 h after inoculation. The expression levels of cell wall genes (β-GAL, PL, PG and PME) showed a general decrease in HT fruit as compared to the control, whereas PAL, CHI, HSP70 and ROS-scavenging genes increased their expression level in HT samples with respect to the untreated ones. Our results show a curative activity of heat on peach inoculated with M. laxa 48 h before treatment. Each analyzed gene proved to be differentially expressed following heat treatment.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Postharvest Biology and Technology - Volume 94, August 2014, Pages 66–73
نویسندگان
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