کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4562979 | 1330738 | 2007 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Physicochemical basis for cosolvent modulation of β-lactoglobulin functionality: Interfacial tension study
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
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چکیده انگلیسی
The impact of neutral cosolvents on the thermal stability of globular proteins in aqueous solutions depends on the nature of the cosolvent, e.g., sorbitol causes a pronounced increase in the thermal denaturation temperature (Tm) of β-lactoglobulin (β-lg), whereas glycerol does not. When a protein unfolds there is a change in the exposed surface area and in the type of molecular interactions that occur at the protein-solvent-cosolvent interface. These changes contribute to the free energy change associated with protein denaturation and depend on cosolvent type. In this study we measured the equilibrium interfacial tensions of aqueous glycerol (0-70% w/w) and sorbitol (0-55% w/w) solutions as a function of temperature to provide insights into the role of the interfacial energy on the thermal stability of β-lg. There was a slight increase in interfacial tension with increasing sorbitol concentration, indicating its preferential exclusion from the oil-water interface. On the other hand, there was an appreciable decrease in interfacial tension with increasing glycerol concentration, indicating its preferential accumulation at the oil-water interface. These changes were largely independent of the measurement temperature (30-80 °C). Our results suggest that sorbitol increases the Tm of β-lg mainly through a steric exclusion effect, whereas glycerol has little effect on Tm because the steric exclusion effect is counter-balanced by a differential interaction effect.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Research International - Volume 40, Issue 8, October 2007, Pages 1098-1105
Journal: Food Research International - Volume 40, Issue 8, October 2007, Pages 1098-1105
نویسندگان
Wanlop Chanasattru, Eric A. Decker, D. Julian McClements,