کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4563535 1628526 2016 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of polyphenols in grape-based nutraceutical products using high resolution mass spectrometry
ترجمه فارسی عنوان
تعیین پلی فنل در محصولات مبتنی بر انگور مبتنی بر با استفاده از اسپکترومتری جرم با وضوح بالا
کلمات کلیدی
مواد مغذی مبتنی بر انگور، ترکیبات پلیفنول، طیف سنجی جرمی، اربیتپور
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
چکیده انگلیسی


• Determination of polyphenols in grape-based nutraceuticals by UHPLC-Orbitrap-MS.
• Determination of all classes of polyphenols in grape in less than 16 min.
• Retrospective analysis to identify other phenolic compounds in grape-based nutraceuticals.
• High variability of resveratrol content in grape-based nutraceuticals.

The detection and quantification of polyphenols in grape-based nutraceutical products were performed using ultra high performance liquid chromatography coupled to single-stage Orbitrap high resolution mass spectrometry (UHPLC-Orbitrap-MS). The method is based on an extraction using a mixture of methanol:water (80:20, v/v), followed by two dilutions (10 or 50 times). The method was validated in terms of linearity, precision and trueness, and limits of detection and quantification ranged from 1 to 10 μg L−1 and 2–50 μg L−1 respectively. High variability of the amount of anthocyanins and flavanols were obtained in the analyzed samples, with values ranging from 2 to 27448 mg kg−1, whereas the resveratrol was only detected in 6 out of 8 samples at concentrations ranging from 3 to 11107 mg kg−1. Furthermore, other polyphenols belonging to other families like dihydrochalcones, flavanones, flavonols, isoflavonoids or phenolic acids (hydroxybenzoic and hydroxycinnamic acids) were quantified. Apart from this, a tentative identification of 8 compounds was performed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: LWT - Food Science and Technology - Volume 71, September 2016, Pages 249–259
نویسندگان
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