کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4754886 | 1361490 | 2017 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Co-expression of glucose-6-phosphate dehydrogenase and acyl-CoA binding protein enhances lipid accumulation in the yeast Yarrowia lipolytica
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The oleaginous yeast Yarrowia lipolytica is a convenient model for investigating lipid biosynthesis and for engineering high lipid accumulated strains. In this organism, the pentose phosphate pathway is the major source of NADPH for lipid biosynthesis. Thus, we over-expressed gene encoding NADP+-dependent glucose-6-phosphate dehydrogenase (ZWF1) in a strain deficient in peroxisome biogenesis. However, this strategy suppressed growth during cultivation under lipogenic conditions and did not significantly increase lipid accumulation. Remarkably, co-expression of gene encoding acyl-CoA binding protein (ACBP), which functions as an intracellular acyl-CoA transporter and acyl-CoA-pool former, restored growth. Co-expression of ZWF1 and ACBP increased the lipid content to 30% of dry cell weight via de novo lipid synthesis. In comparison to wild type, the engineered strain accumulated 41% more lipids with a higher ratio of saturated to unsaturated fatty acids.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: New Biotechnology - Volume 39, Part A, 25 October 2017, Pages 18-21
Journal: New Biotechnology - Volume 39, Part A, 25 October 2017, Pages 18-21
نویسندگان
Evgeniya Y. Yuzbasheva, Elizaveta B. Mostova, Natalia I. Andreeva, Tigran V. Yuzbashev, Ivan A. Laptev, Tatiana I. Sobolevskaya, Sergey P. Sineoky,