Optimized endodextranase-epoxy CIM® disk reactor for the continuous production of molecular weight-controlled prebiotic isomalto-oligosaccharides

- Dextranases were immobilized on CIM disk to produce isomalto-oligosaccharides (IMOs).
- The immobilization yield of enzymes was 45% for a specific activity of 3.26 U mg−1.
- The enzymatic reactor showed 80% of residual activity after 5000 column volumes.
- Surface response methodology was performed to optimize the reactor.
- The prebiotic activity of IMOs was close to commercialized fructo-oligosaccharides.

An epoxy-activated monolithic Convective Interaction Media (CIM®) disk was used for the immobilization of endodextranase D8144 from Penicillium sp. (EC 3.2.1.11) in order to produce on-line isomalto-oligosaccharides (IMOs) from Dextran T40. Enzymatic parameters, molecular weight of IMOs and performance of the IMmobilized Enzymes Reactor (IMER) were investigated. The immobilization yield of enzymes was about 45.3% (w/w), and the real specific activity close to 3.26 U mg−1. The Km values did not significantly change between free (12.8 g L−1) and immobilized enzymes (14.2 g L−1), due to the absence of diffusional limitation. The IMER system presented more than 80% of its residual activity after 5000 column volumes, highlighting the high stability of the immobilized endodextranases. Response surface methodology was used to enhance the performance of the IMER. Depending on dextran concentrations and flow rates, specific patterns of IMOs distributions were observed during the enzymatic hydrolysis. Finally, prebiotic activity was also investigated on IMOs produced by medium conditions (flow rate 0.3 mL min−1 and dextran concentrations 4% w/w) against Lactobacillus rhamnosus GG (ATCC 53103). Their scores were at least as good as two commercialized fructo-oligosaccharides (FOS), Fibrulose® F97 and Orafti® P95.

90