کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4767835 1424505 2017 35 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Use of protic ionic liquids as adjuvants in PEG-based ATPS for the purification of radish peroxidase
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Use of protic ionic liquids as adjuvants in PEG-based ATPS for the purification of radish peroxidase
چکیده انگلیسی
This work addresses the use of protic ionic liquids (PIL) as adjuvants (5 wt%) in the formation of aqueous two-phase systems (ATPS), based on polyethylene glycol with different molecular weights + ammonium sulfate + water for peroxidase purification from alternatives vegetables as radish peroxidase (Raphanus sativus L.) from horseradish root peroxidase. Protic ionic liquid as a process adjuvant did not extend the biphasic area in the phase diagram, but the phase behavior was modified according to the hydrophobicity of the PIL used and additional interactions between the enzyme and PIL increased the purification factor of the radish peroxidase from ≈2.5 (no ionic liquid) to ≈ 19.2 fold using 2-hydroxyethylammonium propionate as adjuvant in ATPS.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fluid Phase Equilibria - Volume 452, 25 November 2017, Pages 1-8
نویسندگان
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