Modification with DEAE-dextran, an alternative way to prepare anion-exchange monolithic column with lower pressure drop

Poly(methacrylate-co-ethylene dimethacrylate) monolithic column (100 mm × 4.6 mm i.d.) subsequently modified with DEAE-dextran had been prepared for the separation of proteins with anion-exchange mode. The column, which possessed the specific surface area of 5.3 m2/g and immobilized amine group of 9.93 μmol/g media, showed even better permeability of 607 psi at flow rate of 1445 cm/h in comparison to diethylamine modification procedure. The dynamic binding capacity of bovine serum albumin (BSA) was 23.8 mg/g media. Fast separation of lysozyme, hemoglobin and BSA on the column was achieved within 3 min at flow rate of 1445 cm/h.