کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4908188 | 1426594 | 2016 | 6 صفحه PDF | دانلود رایگان |
- A photoelectrochemical AChE biosensor based on semiconductor nanocomposite was developed.
- The combination of ZnO and TiO2 enhanced the photoelectrochemical activity.
- Cd2Â + showed dose-dependent effects on AChE activity.
Acetylcholinesterase (AChE) is a well-known serine hydrolase and its dysfunction could disturb cholinergic neurotransmission which is related to the pathogenesis of neurodegenerative disorders. In this report, a photoelectrochemical biosensor based on metal oxide semiconductor nanocomposite was fabricated to investigate the effect of cadmium ion (Cd2Â +) on the activity of AChE. The photoelectrochemical nanocomposite was prepared by anodic oxidation of titanium (Ti) foil to form titanium dioxide (TiO2) nanotubes (TNs) array followed by cathodic deposition of zinc oxide nanorods (ZnONRs) onto the TNs. AChE was immobilized on the obtained nanocomposites and the biosensor showed enhanced photoelectrochemical response under visible light irradiation. The experimental results showed that Cd2Â + exhibited interesting dose-dependent and time-dependent effects on AChE activity. Specifically, high concentration of Cd2Â + inhibited while low level of Cd2Â + could stimulate the activity of AChE. These findings are of great significance for the study of enzyme activity influenced by metal ions and related pathogenesis investigation of neurodegenerative disorders.
A photoelectrochemical biosensor based on metal oxide semiconductor nanocomposite was developed to investigate the effect of Cd2Â + on AChE activity.90
Journal: Journal of Electroanalytical Chemistry - Volume 781, 15 November 2016, Pages 377-382